CD4 T Lymphocytes Enumeration Techniques

 

 

Annex 2: Programme

 

Monday, 24 November 2003

0830-0900 hrs

Registration

 

0900-0930 hrs

Official opening ceremony

 

1000-1015 hrs

Objectives

Dr Kumari

1015-1030 hrs

Overview of 3 x 5 strategy on ARV Therapy

Dr Jai Narain

1030-1045 hrs

Overview of the WHO CD4 related activities

Dr Vercauteren

1045-1130 hrs

Country reports:

India

Indonesia

Myanmar

Thailand

All participants

1130-1145 hrs

Discussion

 

1145-1200 hrs

Pre-workshop test

Participants

1200-1245 hrs

The role of CD4 T cell counting in HIV/AIDS

Dr Vercauteren

1245-1300 hrs

Discussion

 

1400-1445 hrs

Overview of CD4 T cell enumeration technologies

Dr Jani

1445-1530

Principles of flow cytometry

Dr Pattanapanyasat

1530-1600 hrs

Discussion

 

1615-1700 hrs

Gating strategies and selection of reagents related to lymphocyte subset counting

Dr Pattanapanyasat

1700-1730 hrs

Discussion

 

1730-1745 hrs

Review of day 1

 

Tuesday, 25 November 2003

0900-0920 hrs

Flow cytometer calibration

Dr Jani

0920-0930 hrs

Discussion

 

0930-1015 hrs

Haematology analysers: total leucocyte, lymphocyte counts; differentials

Dr Sathien Sukpanichnant

1015-1030 hrs

Discussion

 

1045-1130 hrs

The concept of PanLeucogating

Dr Jani

1130-1300 hrs

Tour of the Institute

 

Practical exercises

1400-1545 hrs

Flow cytometry: group 1
Alternative technologies: group 2

Dr Pattanapanyasat
Indian Expert

1600-1745 hrs

Alternative technologies: group 1
Flow cytometry: group 2

 

Wednesday, 26 November 2003

0830-0900 hrs

Review of practical experiments

 

0900-0945 hrs

Group work: paper exercises on haematology and flow cytometry

Dr Jani

0940-1015 hrs

Presentation of group work and

 

1015-1030 hrs

Discussion

 

1045-1145 hrs

Group work: How to select and purchase the most appropriate technology

All facilitators

1145-1230 hrs

Presentation of group work discussion

 

1400-1500 hrs

Quality assurance: How to produce reliable results

Dr Jani

1500-1515 hrs

Discussion

 

1515-1545 hrs

Role of stabilizing reagents

Dr Jani

1600-1630 hrs

External quality assessment schemes

Dr Pattanapanyasat

1630-1700 hrs

Maintenance of equipment in the immunophenotyping laboratory

Dr Pattanapanyasat

1700-1715 hrs

Discussion

 

1715-1730 hrs

Review of day 3

 

Thursday, 27 November 2003

0900-0945 hrs

Method comparison

Dr Jani

0945-1000 hrs

Discussion

 

1015-1045 hrs

Safety in the immunophenotyping laboratory

Dr Vercauteren

1045-1100 hrs

Discussion

 

1100-1145 hrs

Group work: identify the main problems/solutions in running a CD4 counting service

 

1145-1230 hrs

Presentation group work and discussion

 

1230-1300 hrs

Practical issues in the management of an immunophenotyping laboratory

Dr Pattanapanyasat

1400-1415 hrs

Post workshop test

 

1415-1530 hrs

Recommendations

 

1530-1600 hrs

Closure

 

 

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