Laboratory Diagnosis of Gonorrhoea

Annex - 2 – Standard Reporting Sheets


Data Collection Sheet for Individual Centres to Report to Regional Co-ordinating Centre

(Fill in one sheet only for each category of population tested and type of susceptibility testing.)

1.      Centre name

Town/City

Country

WHO region

2.      WHO co-ordinating centre: Australia

3.      Patient population tested (please tick Ö )

Unknown [  ]

STD patients [  ]

Screening, case finding [  ]

4.      Briefly describe population tested

5.      Gonococcal strain population tested (please tick Ö )

Consecutive (all isolates) [  ]

Sentinel (systematic samples) [  ]

Random (give details) [  ]

6.      Briefly describe strain population

7.      Method of testing, e.g. SEARO method disc or MIC (if done)

 

Data Recording Sheet

Laboratory

Date: From To

Method of testing: [specify for each antibiotic if not using the SEAR method]

Please enter zone sizes (annular radius mm) under the appropriate category of sensitivity for reference strains selected and test strains. Start a new sheet for each batch.

 

Strain

b-lact

Penicillin

Ciprofloxacin

Nalidixic acid

Ceftriaxone

Spectino.

Tetracycline

Category

 

R*

S/LS**

R

LS

S

R/LS

S

LS

S

R

S

TRNG

not TRNG

ANNULAR
RADIUS (mm)

 

<3

>4

<6

>6

>6

0

>6

<6

>6

0

>6

<1

>2

Reference
strains
WHO A

 

 

 

 

 

 

 

 

 

 

 

 

 

 

WHO B

 

 

 

 

 

 

 

 

 

 

 

 

 

 

WHO C#

 

 

 

 

 

 

 

 

 

 

 

 

 

 

WHO D

 

 

 

 

 

 

 

 

 

 

 

 

 

 

WHO E

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Test  strains

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 * Does not respond to penicillin

** Responds to penicillin treatment

# WHO C to be included with each batch of disc testing

 Summary Reporting Sheet for SEAR GASP Network Laboratories

Laboratory

Date:      From                            To

Antibiotics

Core group

Total  number
of strains
tested

Number of strains per category

b-lactamase

Chromosomal resistance

Positive

Negative

Resistant

Less sensitive

Susceptible

Penicillin

 

 

 

 

 

 

Ciprofloxacin

(or other

quinolone;

specify)

 

 

 

 

 

 

Ceftriaxone

 

 

 

 

 

 

Spectinomycin

 

 

 

 

 

 

Tetracycline

 

TRNG
positive

TRNG
negative

 

 

 

 

 

Additional group

Thiamphenicol/

Chloramphenicol

Kanamycin

Other cephalosporins

Azithromycin

 

 

HO GASP Quality Assurance Programme

1998 QA Programme for WPR and SEARO

 

Batch 1: Neisseria gonorrhoea strains 98 QA 1–6

Date tested                                                   Laboratory

Testing method: Please tick ( )

[    ] MIC mg/L [    ] Disc: (  ) Zone diameter or (  ) Annular radius

[    ] American [    ] non-American: (  ) AGSP or (  ) other

Results: Please enter MIC value (mg/L) or zone size (mm) AND Category of sensitivity for strains 1–6. Categories based on zone sizes and MICs are contained in the Reference Manual. If using AGSP system disc testing, annular radius (mm) replaces zone diameter.

98 QA Strain

Penicillin

Ceftriaxone

Ciprofloxacin

Spectinomycin

Tetracycline

Nalidixic acid

Disc
potency

S/LS/R/PPNG

S or R

S/LS/R

S or R

TRNG or Not TRNG

S or R

1. MIC

 

 

 

 

 

 

Zone Size

 

 

 

 

 

 

Category

 

 

 

 

 

 

  

 

 

 

 

 

 

2. MIC

 

 

 

 

 

 

Zone Size

 

 

 

 

 

 

Category

 

 

 

 

 

 

 

 

 

 

 

 

 

3. MIC

 

 

 

 

 

 

Zone Size

 

 

 

 

 

 

Category

 

 

 

 

 

 

 

 

 

 

 

 

 

4. MIC

 

 

 

 

 

 

Zone Size

 

 

 

 

 

 

Category

 

 

 

 

 

 

 

 

 

 

 

 

 

5. MIC

 

 

 

 

 

 

Zone Size

 

 

 

 

 

 

Category

 

 

 

 

 

 

 

 

 

 

 

 

 

6. MIC

 

 

 

 

 

 

Zone Size

 

 

 

 

 

 

Category

 

 

 

 

 

 

 

 

 

 

 

 

 

Quality Control Chart for Antimicrobial Susceptibility Testing using SEAR GASP Network Disc Method

Control strain: N. gonorrhoeae WHO C

 

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