Guidelines on Standard Operating Procedures for CLINICAL CHEMISTRY

Introduction


Urine is one of the most easily obtained specimens examined in the laboratory, and examination of the urine not only provides information about the functioning of the kidneys and possible abnormalities of the urinary tract, but may also lead to the diagnosis of various systemic diseases of the human body which are reflected by the presence of several substances in the urine.

*     Collection of specimen

 

*     Early morning urine

This is the best urine specimen for routine analysis and is collected soon after the patient awakens. It is usually concentrated and has an acid pH. Casts and cells are poorly preserved in dilute or alkaline urine and traces of dissolved substances such as protein and sugar can be missed if the urine is very dilute.

*     Random urine

This specimen is collected at any time and is convenient for the patient and is suitable for most screening purposes.

*     Preservative used

For routine analysis, no preservative is required but the urine is best examined fresh. Bacterial growth will ruin a specimen if analysis is delayed for more than 3 hours. Refrigeration is the best way to preserve it if analysis is delayed. Refrigeration for more than 24 hours isnot recommended.

*     Container for urine collection

The container used must be thoroughly clean and free from any detergent or disinfectant residue since the oxidants contained in such cleaning agents may cause the test areas for glucose and blood to indicate false positive results. After the urine is collected, the container should preferably be sealed.

 

 

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